Dr. E.Selvarajan has done B.Tech. Industrial Biotechnology from Arunai Engineering College, Anna University, Chennai in 2007 and M.Tech. Industrial Biotechnology from SASTRA University, Tanjore, in 2009. He obtained his PhD from VIT University, Vellore, in 2015. For his PhD research, he Immobilized β-galactosidase from Lactobacillus plantarum VITES07 on ZnO nanoparticles for lactose hydrolysis and characterized. He worked on multiple projects which include enhanced production of α-amylase, staphylokinase, streptokinase and nattokinase, Bioremediation of endosulfan contaminated soil, Fermentative production of extracellular pigment from Streptomyces coelicolor. He has about 30 publications, which include 5 book chapters, 22 original and 3 review papers, etc, with h index of 9 and more than 347 citations (Google scholar). He worked at NIT Calicut for 1 year and then joined SRM University in 2016.
Veena Ramesh, S. Melvin Samuel, Ethiraj Selvarajan, Methods for Characterizing Nanoparticles, CRC Press, 2019. E.Selvarajan, V.Mohanasrinivasan (2019) Nanoimmobilization of β-Galactosidase for lactose free product development. Springer Publisher . K M Gothandam et al. (eds.), Nanoscience and Biotechnology for Environmental Applications, Environmental Chemistry for a Sustainable World 22, https://doi.org/10.1007/978-3-319-97922-9_7 . Biosynthesized silver nanoparticles using Bacillus amyloliquefaciens; Application for cytotoxicity effect on A549 cell line and photocatalytic degradation of p-nitrophenol, Arivalagan Pugazhendhi Melvin S. Samuel, Sujin Jose, E. Selvarajan, Thangavel Mathimani, Journal of Photochemistry and Photobiology B: Biology, 202 (2020), 111642 . Dielectric and Electromagnetic Interference Shielding Properties of Germanium Dioxide Nanoparticle Reinforced Poly (vinyl chloride) and Poly (methylmethacrylate) Blend Nanocomposites. Jenifer Joseph, Kalim Deshmukh, K. Chidambaram, Muhammad Faisal, E. Selvarajan, Kishor Kumar Sadasivuni, M. Basheer Ahamed,S. K. Khadheer Pasha. Journal of Materials Science: Materials in Electronics, 2018 , 29:20172–20188 Biosynthesis of MgO nanoparticles using Lactobacillus sp. and its activity against Human Leukemia cell lines HL-60”. V.Mohanasrinivasan, C. Subathra Devi E. Selvarajan, S. Jemimah Naine. Bionanoscience 2018 Immobilization of β-galactosidase from Lactobacillus plantarum HF571129 on ZnO nanoparticles: Characterization and lactose hydrolysis” E. Selvarajan, V.Mohanasrinivasan, C.Subathra Devi, George Priya Doss. Bioprocess and Biosystem Engineering, 2015, 38(9), 1655-1669 . ”Biosynthesis and characterization of ZnO nanoparticles using Lactobacillus plantarum VITES07” E. Selvarajan, V.Mohanasrinivasan. Materials Letters, 2013, 112, 180-182.
Doctor of Philosophy in Biotechnology – Vellore Institute of Technology University, Tamil Nadu (April 2015). Master of Technology in Industrial Bio-Technology - SASTRA University, Tamil Nadu with CGPA of 9.11/10 and placed in First class with distinction (May 2009). Bachelor of Technology in Industrial Bio-Technology - Arunai Engineering College, Anna University,Chennai with 78% and placed in First class (April 2007).
As a part of my doctoral thesis, I have worked with Zinc Oxide nanoparticle synthesis by bacteria and its developed a method for immobilization of the enzyme Beta galactosidase for development of Lactose free milk produccts. Lactose intolerance is one of the major problems faced by as high as 68% of the world population and India is one of the countries which has a very high incidence of lactose intolerance in its population. Beta galactosidase is hydrolase enzyme that cleaves the glycosidic bonds that are present inside Lactose to produce galactose and alcohol. In this project we considered immobilization of this enzyme onto nanoparticles so that the product we get at the end is free of contamination from the enzyme. Moreover it is very expensive to produce enzymes on a large scale and immobilization of these enzymes onto the nanoparticles can help in efficient revovery of the catalysts, better thermal stability, controlled product formation, high yield and high reactor productivity. The rationale behind the selection of ZnO was the wide variety of literature that was available for perusal. Zinc oxide nanoparticles have been found to have multiple uses like catalysis, gas sensing, cancer treatment, chemical absorbent antibacterial and UV blocking functions and in cosmetic and pharmaceutical industries. Recently ZnO nanoparticles have been employed for immbilation of various enzymes and as it is a Generally regarded as Safe(GRAS) compound licensed by the FDA we decided to select this as the candidate for immbilsation of Beta galactosidase As a part of the project, one of the major challenges was the production of a non toxic and cheaper method for production of ZnO nanoparticles.After going through various background literature,we decided that the probiotic species, Lactobacillus plantarum can be a plausible candidate for synthesis o ZnO nanoparticles. One of the major concerns with the synthesis of ZnO nanoparticle is the cost activity relationship. Most of the known synthesis methods have been observed to be very costly for getting a discernible amount of nanopartilces and may additionaly be toxic for human use.This method of biogenesis from the Lactobacillus plantarum species was much cheaper and is produced in a way that doesnot involve the usage of toxic chemicals. The ZnO nanoparticles that were synthesized this way were also very moderately stable and had a size of 7-19 nm. This stable,inexpensive, non toxic and ecofriendly ZnO nanoparticles served as a good candidate for immobilization of Beta Galactsidase. Although the ZnO nanoparticles was predominantly prepared with the objective of immobilizing Beta galactosidase, the 70 + citations it racked up over the course of 6 years (Google Scholar metrics) shows the impact it had over a wide variety of research. We then went ahead andcharcterised a novel Beta Galactosidase from Lactobacillus plantarum which showed very efficient catalytic ability for lactose hydrolysis. This novel Beta Galactosidase was then finally immobilized ZnO nanoparticles. The immobilized enzyme was seen to retain 70% of its activity even after 7 cycles and seen to retain 85% of its activity after 30 days when stored at 4 degree and ph 6.5. From the study we not only observed good stability of the enzyme but also a higher efficiency of enzyme catalysis. This technology can used in the future in the food industry as a very efficient method for production of lactose free products. I have also worked and guided students in the field of Production of enzymes and other natural products from bacteria and by chemical synthesis. Some of the enzymes and natural products that I have worked with are as follows- Bis(indolyl)Methanes- In this work we produced a seried of Bis(Indolyl)Methanes by a one pot reaction and checked for its antibacterial and anti-inflammatory properties Zinc Tetrafluoroborate-In this work a series of novel dimethyl ((aryl/heteroarylamino) (4-(pyrrolidin-1-yl) phenyl)) methyl phosphonates were synthesized by simple and an efficient one-pot three component Kabachnik- Fields reaction and checked for their anticancer activity Lipase- In this work we charcaterise a novel extracellular lipase from Serratia marcescens Streptokinase- As a part of this work we used random UV mutagenesis for enhaced streptokinase production from Streptococcus equismilis Nattokinase- This work dealt with the enhanced production of the enzyme Nattokinase by UV mutagenesis. Human granulocyte colony stimulating factor- This project dealt with purification of huma granulocyte colony stimulating factor from inclusion bodies in Escherichia coli Staphylokinase- This work was based on extraction of Staphylokinase extracted and purified from Staphylococcus spp. screened from milk samples Ergosterol- As a part of this project we isolated the yeast Saccharomyces cerevisae from grape juice and used it for fermentative production of ergosterols. Dextran- The biocompatible polymer Dextran was produced from the bacteria Leuconostocmesenteroides and its cross linking was studied L-Asparaginase II- In this work we overexpressed and purified a novel L-Asparaginase II from Lactobacillus casei Silver oxide nanoparticles- In this recently published work we synthesized silver nanoparticles from the bacteria Bacillus amyloliquifaciens and its ability to photocatalytically degrade p-nitrophenol was studied along with the cytotoxic effects it had on the A549 cell line. MgO nanoparticles- In this study we biosynthesized MgO nanoparticles from Lactobacillus spp. and studied its cytotoxic effects on the HL-60 human leukemia cell line.
HONORS AND AWARDS
Received “HAR GOBIND KHORANA BEST YOUNG SCIENTIST AWARD” for Biotechnology 2019. Received Young Scientist Award,Bioengineering, AufauInternational Award 2019 Nominated for “Who’s Who in the World 2016” 33rd Edition, Published from Marquis Who’s who in America, since 1899. Best Research scholar award from VIT University for the year 2012 and 2013
University of Wisconsin-Milwaukee, USA Ton Duc Thang University, vietnam
Journal of Cellular Biochemistry 3 Biotech International Journal of Phytoremediation Molecular Biology Reports Asian Journal of Research in Biochemistry Frontiers in Biology Applied Biochemistry and Biotechnology Indian Journal of Biotechnology American Journal of Bioscience African Journal of Biotechnology